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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Important role of the SDF-1/CXCR4 axis in the homing of systemically transplanted human amnion-derived mesenchymal stem cells (hAD-MSCs) to ovaries in rats with chemotherapy-induced premature ovarian insufficiency (POI)

Fig. 7

Effects of hAD-MSCs on ovarian injuries induced by chemotherapy in POI rats after blocking the SDF-1/CXCR4 axis. a Changes in the ovarian tissue were analysed using HE staining (×100 and ×200). b The number of follicles at different stages was counted and compared in the control, POI, hAD-MSCs and hAD-MSCs + AMD3100 groups (n = 10). c Ovarian granulosa cell (GC) apoptosis was tested using the TUNEL assay (×100 and ×400). d The expression levels of Bax, Bcl-2, cleaved-caspase-3, VEGF and VEGFR2 in the ovaries were detected using immunohistochemical staining (×100 and ×400). e–i Semiquantitative analyses of Bax, Bcl-2, cleaved-caspase-3, VEGF and VEGFR2 levels in the ovaries from each group are shown (n = 10). Each dot in the graphs (e–i) represents the value obtained from ten high-power fields (HPFs) randomly chosen from five sections in each group. The bars and error bars in graphs (e–i) indicate the medians and ranges, respectively. Brown cells represent immunostained cells. Representative images are shown. *P < 0.05 and **P < 0.01. Scale bars = 100 μm

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Stem Cell Research & Therapy

ISSN: 1757-6512