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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Genetic correction of concurrent α- and β-thalassemia patient-derived pluripotent stem cells by the CRISPR-Cas9 technology

Fig. 2

PCR and DNA sequence analysis of the corrected hiPSC clones. A After β41-42 homozygous mutation was completely corrected, the repaired clones showed two bands with different sizes in P1/P2 and P3/P4 amplification. B The black box indicates β41-42 deletion in the position of the new 59th codon. The sequencing results showed the β41-42 (-TCTT) heterozygous mutation before and after gene correction. C After the Westmead mutation was repaired, the repaired clones showed 500-bp and 2000-bp bands in PR/PF amplification. D The black box indicates the Westmead mutation was located in the last exon CD122 (CAC > CAG). Sequencing results showed the Westmead mutation (CAS → CAC) before and after gene correction. The P1/P2, P3/P4 and PR/PF were detected by PCR in different gels

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Stem Cell Research & Therapy

ISSN: 1757-6512