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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Human umbilical cord mesenchymal stem cell promotes angiogenesis via integrin β1/ERK1/2/HIF-1α/VEGF-A signaling pathway for off-the-shelf breast tissue engineering

Fig. 2

HUCMSC-CM promoted migration and tubule formation via integrin β1/ERK1/2/HIF-1α signaling pathway in HUVECs. A ATN-161 or 2-MeOE2 was used to pre-treat HUVECs to inhibit integrin β1 or HIF-1α, respectively. B, C HUVECs migration and tubule formation were observed after different treatments. ATN-161 or 2-MeOE2 significantly inhibited hUCMSC-CM-induced migration and tubule formation in HUVECs (***P < 0.001). D Western blot analysis showed hUCMSC-CM-induced VEGF-A expression was significantly suppressed following ATN-161 or 2-MeOE2 treatment in HUVECs (***P < 0.001). E Western blot analysis showed hUCMSC-CM-induced HIF-1α expression was markedly decreased following integrin β1 inhibition in HUVECs (***P < 0.001). F hUCMSC-CM significant promoted ERK1/2 phosphorylation in HUVECs and could be suppressed by inhibition of integrin β1. In contrast, hUCMSC-CM did not affect Akt phosphorylation in HUVECs (***P < 0.001)

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Stem Cell Research & Therapy

ISSN: 1757-6512