Fig. 7

Flow cytometry gating strategy for CD4⁺ and CD8⁺ cells. Lymphocytes were first identified by R1 gate in forward scatter (FSC) and side scatter (SSC) graph. CD4⁺ and CD8⁺ T cells were then measured by means of anti CD4 and anti CD8 antibodies, labeled by FITC and PE respectively; as illustrated in the histogram graphs separately. A and B represent the changes in CD4⁺ and CD8⁺ lymphocyte counts between 5 and 10 days after secretome administration in a 35-year-old male patient, respectively