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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Drug metabolic activity is a critical cell-intrinsic determinant for selection of hepatocytes during long-term culture

Fig. 2

Characteristic analysis of ProliHH during long-term culture. A Gene expression of ProliHH from passage 1 to 21 by qRT-PCR. Data were normalized with the housekeeping gene UBC. The expression level of each gene in PHH was set to 1.0. Error bars indicate the standard deviation (n = 3). B-D Immunocytochemical analysis of ProliHH with antibodies to ALB (B), HNF4A (C) and bile duct marker CK7 (D) at the early and late passages (passage 5 and 21, respectively). Yellow circles indicate binuclear cells (D). E, F. Glycogen storage in ProliHH (passage 3, 13 and 21) by PAS stain with (E) and without (F) diastase digestion. G-i. Expression of the genes for CYP1A2 (G), CYP2B6 (H) and CYP3A4 (I) in ProliHH after exposure to omeprazole (G), phenobarbital (H) or rifampicin (i). The expression level of each gene without any treatment (DMSO) was set to 1.0. Each expression level was calculated from the results of independent (biological) triplicate experiments. Error bars indicate standard error. The student's T test was performed for statistical analysis of two groups. *p < 0.05, **p < 0.01, ***p < 0.001. J CYP3A4 activity of ProliHH (passage 5, 11 and 21). The CYP3A4 activity of PHH was set to 1.0. Error bars represent the standard errors. Expression levels were calculated from the results of independent (biological) triplicate experiments. Error bars indicate the standard error. ***p < 0.001

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