Skip to main content
Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Drug metabolic activity is a critical cell-intrinsic determinant for selection of hepatocytes during long-term culture

Fig. 4

Characteristic analysis of puromycin-selected ProliHH during long-term culture. A Gene expression by qRT-PCR in puromycin-treated ProliHH from passages 1 to 25. Data were normalized with the housekeeping gene UBC. The expression level of each gene in PHH was set to 1.0. Error bars indicate the standard deviation (n = 3). B Gene expression by qRT-PCR in puromycin-treated ProliHH at passages 4, 11 and 17 by qRT-PCR. Puromycin-treated ( +) and non-treated (-) ProliHH at passages 4, 11 and 17 were compared. The data were normalized with the housekeeping gene UBC. The expression level of each gene in PHH was set to 1.0. Error bars indicate the standard deviation. Expression levels were calculated from the results of independent (biological) triplicate experiments. Student's T test was performed for statistical analysis of two groups. *p < 0.05, **p < 0.01, ***p < 0.001. C-E Immunocytochemical analysis of puromycin-treated ProliHH with antibodies to ALB (c), HNF4A (D) and bile duct marker CK7 (E) at the early and late passages (passage 5 and 25, respectively). Yellow circles indicate binuclear cells (E). F, G Glycogen storage in puromycin-treated ProliHH (passage 5, 13 and 25). (F) PAS stain. (G) PAS stain with diastase digestion. H-J Expression of the genes for CYP1A2 (H), CYP2B6 (I) and CYP3A4 (J) in puromycin-treated ProliHH after exposure to omeprazole (H), phenobarbital (I) or rifampicin (J). The expression level of each gene without any treatment (DMSO) was set to 1.0. Each expression level was calculated from the results of independent (biological) triplicate experiments. Error bars indicate standard error (n = 3). The student's T test was performed for statistical analysis of two groups. *p < 0.05, ***p < 0.001. K CYP3A4 activity of ProliHH (passage 5, 11 and 25). The CYP3A4 activity of PHH was set to 1.0. Error bars represent standard errors (n = 3). Expression levels were calculated from the results of independent (biological) triplicate experiments. Error bars indicate the standard error. *p < 0.05, ***p < 0.001

Back to article page