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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Drug metabolic activity is a critical cell-intrinsic determinant for selection of hepatocytes during long-term culture

Fig. 6

Spheroid formation induces hepatic maturation. A–D Histology of spheroids generated from non-treated ProliHH (Puromycin (-)) and puromycin-treated ProliHH (Puromycin ( +)) at early passage (A passage 5) and late passage (B passage 21 or 25). A, B: HE stain. C, D PAS stain (PAS) and PAS stain with diastase digestion (PAS/D). E, F Immunohistochemistry of spheroids generated from non-treated ProliHH (Puromycin (-)) and puromycin-treated ProliHH (Puromycin ( +)) at early passage (upper: passage 5) and late passage (lower: passage 21 or 25). E ALB (Green), CK7 (Red) and Merge (ALB, CK7 and DAPI). F CYP3A4 (Green), MRP2 (Red) and Merge (CP3A4, MRP2 and DAPI)

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Stem Cell Research & Therapy

ISSN: 1757-6512