Fig. 4

The in vivo distribution of intravenously injected sEVs in different organs in EAU and naive mice was examined using confocal microscopy. A Representative confocal images of PKH-26 immunofluorescence in the eyeballs of EAU and naive mice, which were i.v. injected with sEV-N (100 μg) 24 or 72 h prior. B, C. Representative confocal images of PKH-26 immunofluorescence in the lymph nodes and spleen of EAU and naive mice, which were i.v. injected with sEV-N (100 μg) 24 or 72 h prior. D–F Representative confocal images of PKH-26 immunofluorescence in the liver, kidney, and hearts of EAU and naive mice, which were i.v. injected with sEV-N (100 μg) 24 h prior. G-H. The fluorescence intensity was measured by integrated density using ImageJ software. Scale bar = 150 μm. Mean ± SD, n = 3 per group, one-way ANOVA test. ***P < 0.01, *P < 0.05