Skip to main content
Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Long non-coding RNA SNHG5 promotes the osteogenic differentiation of bone marrow mesenchymal stem cells via the miR-212-3p/GDF5/SMAD pathway

Fig. 2

YY1 induces SNHG5 expression. a Diagram of the SNHG5 promoter and location of the primers. Positions marked are relative to the TSS. b ChIP-qRT-PCR show the interaction between YY1 and the SNHG5 promoter in hBMSCs (n = 3). IgG was used for normalization. c The efficiency of transient transfection of si-YY1-1, si-YY1-2, and si-YY1-3 by western blotting (n = 3). Histograms show the quantification of band intensities. β-actin was used for normalization relative to the si-NC group. d The efficiency of transient transfection of si-YY1-1, si-YY1-2, and si-YY1-3 by qRT-PCR (n = 3). GAPDH was used for normalization relative to the si-NC group. e Relative expression of SNHG5 after downregulation or upregulation of YY1 by qRT-PCR (n = 3). GAPDH was used for normalization. (*p < 0.05; **p < 0.01). ChIP, Chromatin immunoprecipitation; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; hBMSCs, human bone marrow mesenchymal stem cells; qRT-PCR, quantitative reverse transcription-polymerase chain reaction; TSS, transcriptional start site; SNHG5, small nucleolar RNA host gene 5; YY1, Yin Yang 1

Back to article page