Fig. 1

The proportion of EpSCs in the normal epidermis was unaffected by DETCs deficiency. A–C Normal epidermis was isolated from age- and sex-matched WT and Tcrδ^−/− mice. The proportions of CD49f^briCD71^dim cells (A) and K15⁺ cells (B) were analyzed by FACS. C The morphology (left panel) and number per visual field (right panel) of K15⁺ cells (green) were analyzed by means of immunofluorescence (IF). K14⁺ cells (blue) and epidermal T cells (red) are also shown in the images. Scale bar: 100 µm. D Paraffin sections of skin tissues from 8-week-old WT and Tcrδ^−/− mice that had been labeled with BrdU after birth. BrdU⁺ cells in these tissues were detected by means of immunohistochemistry (IHC). Scale bar: 100 µm. The arrow indicates positively stained cells (tan staining). All data are representative of at least three independent experiments and represent mean ± SD of indicated number of mice per group. The p value was calculated by Student’s unpaired t test (A–C) (^nsp > 0.05)