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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Dendritic epidermal T cells secreting exosomes promote the proliferation of epidermal stem cells to enhance wound re-epithelialization

Fig. 2

DETCs increased the proportion of EpSCs in the epidermis around the wound. A–C Full-thickness wounds were generated in age- and sex-matched WT and Tcrδ−/− mice using a sterile 6-mm punch tool on day 0. Epidermal tissues around the wound were isolated on day 3 after injury. The proportions of CD49fbriCD71dim cells (A) and K15+ cells (B) were detected by FACS. C The morphology (left panel) and number per visual field (right panel) of K15+ cells in the epidermis around wounds in WT and Tcrδ−/− mice on day 3 after injury were analyzed by means of IF. Scale bar: 100 µm. D Paraffin sections of wounded skin tissues on day 3 post-wounding from 8-week-old WT and Tcrδ−/− mice that had been labeled with BrdU after birth. BrdU+ cells in these tissues were detected by means of IHC. The arrow indicates positively stained cells (tan staining). Scale bar: 100 µm. E–G Cultured DETCs (1 × 105 cells/wound) or PBS was added to the wound bed of Tcrδ−/− mice. Three days later, epidermal tissues around the wound were collected. The proportions of CD49fbriCD71dim cells (E) and K15+ cells (F) were detected by FACS. G The morphology (left panel) and number per visual field (right panel) of K15+ cells were analyzed by means of IF. Scale bar: 100 µm. H Wounded skin tissues on day 3 post-wounding from 8-week-old Tcrδ−/− mice that had been labeled with BrdU as previously described were used to detect BrdU with immunohistochemical staining. The arrow indicates positively stained cells (tan staining). Scale bar: 100 µm. All data are representative of at least three independent experiments and represent mean ± SD of indicated number of mice per group. The p value was calculated by Student’s unpaired t test (A–C, E–G) (**p < 0.01, ***p < 0.001)

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