Fig. 10

Neonatal exposure to DES treatment affected paternally imprinted genes IGF2-H19 (a) and DLK-MEG-3 (b). DNA methylation enzymes expression was dysregulated (c) and transcripts specific to tumor suppressor (Pten) was not affected (d). Dmrt1, Ezh-2 and cell cycle inhibitor specific transcript p57kip-2 was downregulated (d). Erβ was increased sevenfold (e) and it was interesting to note that alternately spliced Fshr3 is > 40-fold upregulated in DES tumor testes (f). The relative expression level was represented as the fold difference to the value of control taking as 1 and shown as the mean ± S.E. from at least four independent experiments performed on age matched vehicle-treated control and DES-exposed group. *P < 0.05 compared to control