Fig. 2

Neonatal exposure to DES resulted in small-sized atrophied testes. Atrophied testes showed reduced spermatogenesis. The seminiferous tubules were empty and with a dilated lumen (a, b). Spermatid retention was observed (b, i). Spermatids are pulled down to basal Sertoli cells cytoplasm (asterisk). Darkly stained, spermatogonial cells were prominent along the basement membrane (i). Complete histo-architecture was lost (e, f) and there were no germ cells in seminiferous tubules (g). Epididymis was completely devoid of sperm (h). Disordered germ cells arrangement was observed. Interstitial hyperplasia was prominent (c, d) and giant cells were observed at places (arrow). These cells are formed due to germ cell degeneration. Scale: 20 µm