Fig. 2

Corticosterone-induced apoptosis of cortical neurons is caused by ER stress. A ER stress or apoptosis-related proteins was measured by immunoblot in corticosterone or ISRIB-treated cells. B Quantification of CHOP protein levels shown in A. The relative expression of protein was normalized to β-actin. n = 4 C Quantification of cleaved caspase3/total caspase-3 protein levels shown in A. n = 4 D Quantification of Bcl2 protein levels shown in A. The relative expression of protein was normalized to β-actin. n = 4 E Quantification of Bax protein levels shown in A. The relative expression of protein was normalized to β-actin. n = 4 Statistical significance was determined by ANOVA with Bonferroni correction test. Data are shown as relative changes versus controls. *p < 0.05; **p < 0.01 and ***p < 0.001