Fig. 4

The protective effect of A-EVs against corticosterone-induced apoptosis in cortical neurons. A Representative fluorescence images of TUNEL staining in corticosterone with or without A-EVs-treated primary cultured cortical neurons. Scale bar = 50 μm B TUNEL-positive cells/nuclei by image were quantified. n = 4 C Cell viability was measured after treatment in corticosterone with or without A-EVs-treated for 24 h. n = 6 D Apoptosis-related proteins was measured by immunoblot in corticosterone or A-EVs-treated cells. E Expression of cleaved caspase3/total caspase-3 protein level was quantified. n = 4 F Quantification of Bcl2 protein levels. n = 4 G Quantification of Bax protein levels. n = 4. The expression of protein was normalized to β-actin. Statistical significance was determined by ANOVA with Bonferroni correction test. Data are shown as relative changes versus controls. *p < 0.05; **p < 0.01 and ***p < 0.001