Fig. 1

Adipogenesis and osteogenesis of human BMSCs. a Oil Red O and Alizarin Red S staining for adipogenic and osteogenic differentiated BMSCs, respectively, on day 0, 7 and 14. b Distribution of surface markers of BMSCs. c Expression of marker genes. d Immunofluorescence staining of marker proteins. DAPI were used to indicate nucleus. Statistically significant differences of genes between multiple time points versus day 0 were performed with Bonferroni’s multiple comparisons test after one-way ANOVA test. Scale bars = 200 μm in pannel a. Scale bars = 75 μm in pannel d. All data are presented as means ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001