Fig. 1

Regulation of the lineage of AT2 cells by prkg2 in 3D organoids. A Differential interference contrast (DIC) images of AT2 organoids on days 6 and 12. Scale bar, 1 mm. B Statistical analysis of colony number count of wt and prkg2^−/− AT2 between days 4 and 12 (n = 6 wells, two wells per mouse, three mice per group). C Representative images of immunofluorescence stained AT2 organoids on day 12. AT1 (red) and AT2 (green) cells were stained with PDPN and SFTPC antibodies, respectively. Nuclei were counterstained with Hoechst (blue). Dashed white lines indicate the “air space” of spheroids. Scale bar, 50 μm. D AT1 and AT2 cell counts (n = 10 organoids, 1–2 organoids per well, two wells per mouse, three mice per group). E AT1:AT2 ratio (n = 10 organoids, 1–2 organoids per well, two wells per mouse, three mice per group). F Representative images of EdU⁺ cells. Scale bar, 50 μm. G Percentage of EdU⁺ cells (n = 10 organoids, 1–2 organoids per well, two wells per mouse, three mice per group). Data are represented as ± SEM, *p < 0.05 and **p < 0.01 vs. wt by two-tailed Student's t-test