Fig. 2

PKA blockade modifies the regulatory effects of prkg2 on AT2 fate. A Representative DIC images of AT2 organoids treated with PKA inhibitor H89 for 8 days. Scale bar, 1 mm. B Colony number on day 8 (n = 6 wells, two wells per mouse, three mice per group). C Representative images of confocal images. Scale bar, 50 μm. D Total epithelial cells (n = 4 organoids, 1–2 organoids per well, one well per mouse, three mice per group). E AT1:AT2 ratio (n = 4 organoids, 1–2 organoids per well, one well per mouse, three mice per group). F Representative images of EdU-stained organoids. Organoids were labeled with EdU (green) and DAPI (blue). Scale bar, 50 μm. G Statistical analysis of the percentage of EdU⁺ cells in organoids (n = 10 organoids, 1–2 organoids per well, two wells per mouse, three mice per group). Data are represented as ± SEM, *p < 0.05, **p < 0.01, and ***p < 0.001 vs. controls by two-tailed Student's t-test