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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Ascorbic acid 2-glucoside preconditioning enhances the ability of bone marrow mesenchymal stem cells in promoting wound healing

Fig. 4

AA2G-treated BMSC CM promoted wound healing in vitro. a Scratch assay showed the migration capacities of NIH-3T3 cells treated with PBS, BMSC CM, and AA2G-treated BMSC CM for 12 h, respectively. Bar = 120 µm (n = 3). P < 0.0001 based on one-way ANOVA. b Images and quantitative analysis of transmigrated NIH-3T3 cells (stained with crystal violet) are shown. Bar = 120 µm (n = 3). P < 0.01 based on one-way ANOVA. c IF staining of TGF-β for NIH-3T3 treated with the above different CM respectively. Bar = 120 µm. d Hydroxyproline content in NIH-3T3 cells after different CM treatment for 24 h (n = 7). P < 0.01 based on one-way ANOVA. e qRT-PCR was used to detect the expression of collagen-related genes (MMP-3, MMP-9) in NIH-3T3 treated with different CM (n = 3). P < 0.05 based on one-way ANOVA

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Stem Cell Research & Therapy

ISSN: 1757-6512