Fig. 7

AA2G treatment increased the demethylation of BMSCs. a A volcano plot of differentially expressed genes (DEGs). Upregulated DEGs were represented in red and downregulated DEGs were represented in green. b After AA2G treatment, the differential genes were mainly concentrated in the biological process, especially in the cell cycle process, meiosis, and DNA methylation was involved in gamete generation. c Concentration levels of 5-hmc in BMSCs with or without AA2G treatment (n = 9). P < 0.0001 based on t-test. d Representative images of 5-hmc IF staining showed the demethylation of BMSCs and AA2G-treated BMSCs. e Western blot analysis showed the TET2 and VEGF protein expression of BMSCs and AA2G-treated BMSCs. f Western blot analysis showed the AKT, p-AKT, PIK3CA, and p-PI3K protein expression of BMSCs and AA2G-treated BMSCs. g Western blot analysis showed the AKT and p-AKT protein expression of BMSCs treated with AKT inhibitor. h Quantitative analysis of TET2 protein expression of BMSCs (n = 3). P < 0.05 based on t-test. i Quantitative analysis of VEGF protein expression of BMSCs (n = 3). P < 0.05 based on t-test. j Quantitative analysis of p-AKT protein expression of BMSCs (n = 3). P < 0.05 based on t-test. k Quantitative analysis of p-PI3K protein expression of BMSCs (n = 3). P < 0.05 based on t-test. l Quantitative analysis of p-AKT protein expression of AKT inhibitor-treated BMSCs (n = 3). P < 0.01 based on t-test