Table 1 Primer sequences for reverse transcription quantitative PCR
cDNA | Upstream primer | Downstream primer |
|---|---|---|
IL-1β | F: 5′-TCCAGGATGAGGACATGAGCAC-3′ | R: 5′-GAACGTCACACACCAGCAGGTTA-3′ |
iNOS | F: 5′-CAAGCTGAACTTGAGCGAGGA-3′ | R: 5′-TTTACTCAGTGCCAGAAGCTGGA-3′ |
CD163 | F: 5′-ACTTCAGAATCACATCATGGCACA-3′ | R: 5′-TCGTCGCTTCAGAGTCCACAG-3′ |
CD206 | F: 5′-AGAGCTGGCGAGCATCAAGAG-3′ | R: 5′-TTCCATAGGTCAGTCCCAACCAA-3′ |
Nos2 | F: 5′-CCTGCTTTGTGCGAAGTGTC-3′ | R: 5′-CCCAAACACCAAGCTCATGC-3′ |
Ptgs2 | F: 5′-AGCCAGGCAGCAAATCCTT-3′ | R: 5′-GGGTGGGCTTCAGCAGTAAT-3′ |
TNF-α | F: 5′- CGGGCAGGTTCTACTTTGGAG-3′ | R: 5′-ACCCTGAGCCATAATCCCCT-3′ |
Arg1 | F: 5′-AGACCACAGTCTGGCAGTTG-3′ | R: 5′-TGTCAGTGTGAGCATCCACC-3′ |
Chil3 | F: 5′-TGTTCTGGTGAAGGAAATGCGTA-3′ | R: 5′-AGTAGCAGCCTTGGAATGTCTT-3′ |
Retnla | F: 5′-CCCTTCTCATCTGCATCTCCC-3′ | R: 5′-AGTGGAGGGATAGTTAGCTGG-3′ |
TGF-β1 | F: 5′-AGGGCTACCATGCCAACTTC-3′ | R: 5′-CCACGTAGTAGACGATGGGC-3′ |
GAPDH | F: 5′-GCACCGTCAAGGCTGAGAAC-3′ | R: 5′-TGGTGAAGACGCCAGTGGA-3′ |