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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: A transgenic mouse embryonic stem cell line for puromycin selection of V0V interneurons from heterogenous induced cultures

Fig. 2

Determining puromycin selection window based on PAC and Evx1 expression. A Evx1 mRNA expression in day 8, 9, 10, and 11 V0V IN induction cultures derived from RW4 or Evx1-PAC mESCs. N = 7–8. B ICC image analysis showing the percentage of cells expressing V0V IN-markers in RW4- and Evx1-PAC-derived day 11 V0V IN cultures. N = 5. C PAC mRNA expression in day 8, 9, 10, and 11 V0V IN induction cultures derived from RW4 or Evx1-PAC mESCs. N = 5–8. D Representative images of unselected cultures and cultures selected with 4 µg/ml puro in Evx1-PAC mESCs and Evx1-PAC-derived V0V INs. The V0V IN induction was selected on day 10-11 and imaged on day 13. Scale bar is 50 µm. E Percentage of surviving cells after selection with 4 µg/mL puro from day 9–10, day 10–11, or day 11–12, as determined by LIVE/DEAD image analysis (calculated relative to surviving unselected cultures) 2 days after staining (day 12, day 13, or day 14, respectively). N = 6. For A–C, E, error bars are S.E.M. For A and C, significance is indicated as follows: *** denotes p < 0.001 relative to other time points of the same cell line, **** denotes p < 0.0001 relative to other time points of the same cell line, ### denotes p < 0.001 relative to the same time point for the other cell line, #### denotes p < 0.0001 relative to the same time point for the other cell line

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