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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: A transgenic mouse embryonic stem cell line for puromycin selection of V0V interneurons from heterogenous induced cultures

Fig. 4

Long-term selected Evx1-PAC mESC-derived V0V IN cultures express mature neuronal markers and synaptic markers. Representative images of selected cultures stained by ICC. A, B Glutamatergic cell type marker VGLUT2 (green), post-mitotic neuronal marker NeuN (red), and dendritic marker MAP2 (blue). C, D Cholinergic cell type marker VAChT (green), pre-synaptic marker Bassoon (red), and dendritic marker MAP2 (blue). Hoechst is shown in montage images. Scale bars are 50 µm. For each condition, magnified images (Ai, Bi, Ci, Di) are shown adjacent to their derived images to facilitate viewing details. For A and C, cultures were examined at day 16, and for B and D, cultures were examined at day 22

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