Fig. 5
Bursting patterns of networks formed from combined, selected V0V INs, V2a INs, and MNs. A–D Bursting patterns of selected IN and MN cultures on day 26 of Evx1-PAC mESC-derived V0V IN cultures which were surmised to contain glia: A V2a IN, B MN:V2a IN co-cultures, C MN:V0V IN co-cultures, D MN:V2a IN:V0V IN co-cultures. A 10 s time period is shown, and boxes within plots represent Axion’s software indication of a network burst. E–H Bursting patterns of co-cultures containing day 26 Evx1-PAC mESC-derived V0V INs with little glial presence: E, F MN:V0V IN co-culture, G, H MN:V2a IN:V0V IN co-culture. F and H are snippets from E and G, respectively. For E and G, a 15 s time period is shown. In F and H, blue bars show Axion’s software indication of bursts versus black bars which are spikes without bursting. Some semblance of alternation can be detected in both co-culture conditions