Fig. 6
Activity and network metrics of selected co-cultures of V0V INs, V2a INs, and MNs. A–D Metrics of different populations at day 22, 26, or 30 of V0V IN culture. Activity metrics include A mean firing rate (MFR) in Hz, B spikes per burst, C burst duration in seconds, and D burst frequency in Hz. Network metrics include E number of network bursts, F number of spikes per network burst, and G network burst duration in seconds. Synchrony metrics include H the synchrony index, a unitless measurement indicating perfect synchrony at 1 and perfect asynchrony at 0. A legend shows symbols for each population alone, double populations, and all three cell types together, both in the culture with (open symbols, +glia) and without (closed symbols) glia: MN—blue circles, V2a IN—green squares, V0V IN—red triangles, MN:V2a IN—cyan hexagons, V2a IN:V0V IN—orange diamonds, MN:V0V IN—magenta hexagons, MN:V2a IN:V0V IN—gray/crossed squares