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Table 2 Primary antibodies used for ICC and flow cytometry

From: A transgenic mouse embryonic stem cell line for puromycin selection of V0V interneurons from heterogenous induced cultures

Target

Final concentration or dilution used

Source, product number

βIII tubulin

1 µg/mL

Biolegend, 802001

βIII tubulin

1 µg/mL

Biolegend, 845501

Chx10

0.2 µg/mL

Santa Cruz Biotechnology, Inc., sc-374151

Dmrt3

2 µg/mL

Thermo Fisher Scientific, BS-4264R

En1

2 µg/mL

Developmental Studies Hybridoma Bank, 4G11

Evx1

0.5 µg/mL

Developmental Studies Hybridoma Bank, 99.1-3A2a

Hb9

2 µg/mL

Developmental Studies Hybridoma Bank, 81.5C10

Lim1

5 µg/mL

Developmental Studies Hybridoma Bank, 4F2

MAP2

1:1000 dilution

MilliporeSigma, AB5622

NeuN

1:50 dilution

MilliporeSigma, MAB377

Olig2

1:500 dilution

MilliporeSigma, MABN50

VAChT

5 µg/mL

Thermo Fisher Scientific, MA5-27662

VGLUT2

1:3000 dilution

MilliporeSigma, AB2251-I