Table 3 Secondary antibodies used for ICC and flow cytometry
Targeta | Conjugated fluorophore | Final concentrationb (µg/mL) | Product numberc |
|---|---|---|---|
Guinea pig IgG | Alexa Fluor 488 | 4 | A-11073 |
Mouse IgG1 | Alexa Fluor 488 | 2, 4 | A-21121 |
Mouse IgG1 | Alexa Fluor 647 | 4 | A-21240 |
Mouse IgG2a | Alexa Fluor 488 | 2, 4 | A-21131 |
Mouse IgG2a | Alexa Fluor 555 | 4 | A-21137 |
Mouse IgG2a | Alexa Fluor 647 | 2, 4 | A-21241 |
Mouse IgG2b | Alexa Fluor 488 | 2 | A-21141 |
Rabbit IgG | Alexa Fluor 488 | 2, 4 | A-11008 |
Rabbit IgG | Alexa Fluor 647 | 2, 4 | A-21244 |