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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Chaperone-mediated autophagy plays an important role in regulating retinal progenitor cell homeostasis

Fig. 1

mNRPC proliferation was greatly inhibited after IFITM3 knockdown for 48 h. A DimPlot showing the cell types in the murine retina during the embryonic stage and postnatal stage. B FeaturePlot showing the expression and distribution of IFITM3 at E11, E12, E14, E16, P0, P2, P8 and P14. CE Immunofluorescence assays showed decreased expression of IFITM3 in mNRPCs. Magnification: × 400; scale bar: 50 μm. F qRT–PCR showed decreased expression of IFITM3 in mNRPCs. G The results of WB assays were consistent with the qPCR results. H The CCK-8 assay was used to assess cells after IFITM3 knockdown for 48 h. Data are presented as the mean ± SD (n = 3). *P < 0.05; **P < 0.01 (one-way ANOVA and Sidak’s multiple comparisons test). I Cell division-related protein expression was decreased in IFITM3-knockdown cells, as determined by WB assays. The results are representative of at least three independent experiments, and representative blots are shown. Abbreviation: BC, blank control; SC, scramble control

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