Skip to main content
Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Cell-free fat extract attenuates osteoarthritis via chondrocytes regeneration and macrophages immunomodulation

Fig. 4

CEFFE attenuated the differentiation of Raw 264.7 cells from M0 to M1 macrophages. A, D Quantification of CD86-positive cells by flow cytometry. A significant decline in the proportion of M1 macrophages was observed after treatment with CEFFE. B, E Quantification of CD206 positive cells by flow cytometry. No obvious change was observed in the ratio of M2 cells. C, F Immunofluorescence staining of CD86 and bright-field pictures of Raw 264.7 cells. The CEFFE-treated group showed a declining tendency and morphological changes compared with the LPS + IFN-γ group. G Quantification of IL-1β, IL-6, iNOS, and TNF-α mRNA expression by qRT-PCR. The CEFFE-treated group showed a significant dose-dependent decrease compared with the LPS + IFN-γ group. H Quantification of ARG, IL-10, CD206, and TGF-β mRNA expression by qRT-PCR. The CEFFE-treated group showed a slight upward trend compared with the LPS + IFN-γ group. Scale bars are noted on the right bottom corner of each picture. Data represent the mean ± standard deviation (n = 3 per group). *p < 0.05; **p < 0.01; ns, no significant difference between groups

Back to article page