Fig. 6

CEFFE reduced the intracellular production of ROS in RAW 264.7 cells. A, B Fluorescence microscopy observation and the qualification of mean fluorescence intensity. C, D Flow cytometry showed that CEFFE counteracted the intracellular production of ROS induced by LPS + IFN-γ in a dose-dependent manner. E Intracellular NO was measured using a nitric oxide assay kit. Scale bars are noted on the right bottom corner of each picture. Data represent the mean ± SD (n = 3 per group). *p < 0.05; **p < 0.01; ns, no significant difference between groups