Fig. 1
From: Histone chaperone HIRA complex regulates retrotransposons in embryonic stem cells
Depletion of HIRA complex members in ESCs. a qPCR analysis of the expression of Hira, Ubn2/1, Asf1a, and Cabin1 after transfected with control shRNA and shRNAs against Hira, Ubn2/1, Asf1a, and Cabin1. The results were normalized to Gapdh. Data are represented as mean ± s.e.m. (n = 3 independent experiments). ***p < 0.001 in Student’s t test. b Western blot analysis of the indicated proteins in HIRA members and Asf1a-depleted ESCs. Actin was included as a loading control. c–g qPCR analysis of the expression level of pluripotency genes (Oct4, Sox2, and Nanog) in HIRA members Hira (c), Ubn2 (d), Ubn1 (e), Asf1a (f), and Cabin1 (g)-depleted ESCs. The results were normalized to Gapdh. Data are represented as mean ± s.e.m. (n = 3 independent experiments). ns: non-significant, *p < 0.05 in Student’s t test. h Western blot analysis of the expression level of pluripotency genes (Oct4, Sox2, and Nanog) in HIRA members and Asf1a-depleted ESCs. Actin was included as a loading control