Fig. 7
From: Histone chaperone HIRA complex regulates retrotransposons in embryonic stem cells
Hira and Ubn2 regulate H3.3 deposition. a ChIP-qPCR analysis of H3.3 binding on three families of ERVs (ERV1: MMERGLN_LTR; ERV2: IAPLTR2b and RLTR10D; ERV3: MERVL and MT2). **p < 0.01; ***p < 0.001 in Student’s t test. b, c ChIP-qPCR analysis showed that the binding effects of H3.3 on ERV1 and ERV3 were significantly attenuated in Ubn2-depleted ESCs (b), and the binding effect on ERV2 family members (IAPLTR2b and RLTR10D) was weakened after Hira depletion (c). Data are represented as mean ± s.e.m. (n = 3 independent experiments) for the above ChIP-qPCR results. ns: non-significant, **p < 0.01; ***p < 0.001 in Student’s t test