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Fig. 9 | Stem Cell Research & Therapy

Fig. 9

From: PCL/Col I-based magnetic nanocomposite scaffold provides an osteoinductive environment for ADSCs in osteogenic cues-free media conditions

Fig. 9

Effects of the MNPs + PCL/Col I and PCL/Col I scaffolds on the osteogenic differentiation of ADSCs seeding on scaffolds. a–c Gene expressions of COL1, OCN and Runx2 by qRT-PCR. d–h Protein level of Col I, OCN, Runx2, BMP2, and ON proteins by Western blot analysis. Both of assays performed on seeded ADSCs at 21 days of incubation in osteo and osteo cues-free media conditions. Data are the representative of three independent experiments. One-Way ANOVA with Tukey post hoc analysis. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001. Negative control (ADSCs alone whiteout osteo media); Positive control (Osteoblast cells)

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