Name |  | Sequence | Product size (bp) | Annealing temperature (℃) |
---|---|---|---|---|
Subcloning PCR | ||||
PGK-Neo | Forward | TGGGGCGGGATCTGTAGGGCGCAGTA | 2886 | 64 |
Reverse | AACTAGATAACTTCGTATAGCATACATTATACGAAGT | Â | Â | |
AAVS1 homology arm/AmpR | Forward | ACCTGCAGACTAGGGACAGGATTGGT | 5624 | 64 |
Reverse | ACAGATCCCGCCCCACTGTGGGGTGGA | Â | Â | |
CAG-IRES-EGFP | Forward | CGAAGTTATCTAGTTATTAATAGTAATCAATTACGGGG | 3614 | 62 |
Reverse | CCCTAGTCTGCAGGTCGAGGGATCT | Â | Â | |
Human HCN4 | Forward | CAAAGAATTCCTCGAGACCATGGACAAGCTGCCGCCGTC | 3647 | 64 |
Reverse | CCGCGATATCCTCGAGTCATAGATTGGATGGCAGTTTGGAGC | Â | Â | |
Flanking PCR | ||||
Primer 1 | Â | TGGGCTTTGCCACCCTATGCTGACA | Â | 64 |
Primer 2 | Â | ACAAGCAGAAGAACGGCATCAAGGTGAA | Â | Â |
Primer 3 | Â | ACCAACCATCCCTGTTTTCCTAGGACTGA | Â | Â |
qPCR | ||||
GAPDH | Forward | CAACGACCACTTTGTCAAGCTC | 144 | 60 |
Reverse | TCTCTTCCTCTTGTGCTCTTGC | Â | Â | |
HCN4 | Forward | GGTGTCCATCAACAACATGG | 66 | 60 |
Reverse | GCCTTGAAGAGCGCGTAG | Â | Â | |
MYL2 | Forward | GTGCTGAAGGCTGATTACGTTC | 121 | 60 |
Reverse | TGTAGTCCAAGTTGCCAGTCAC | Â | Â | |
NKX2-5 | Forward | GTCCCCTGGATTTTGCATTCAC | 100 | 60 |
Reverse | ATAATCGCCGCCACAAACTCTC | Â | Â | |
NR2F2 | Forward | TCGCCTTTATGGACCACATACG | 149 | 60 |
Reverse | TTCCACATGGGCTACATCAGAG | Â | Â | |
SCN5A | Forward | AGAAGATGGTCCCAGAGCAATG | 131 | 60 |
Reverse | AATCTGCTTCAGAACCCAGGTC | Â | Â | |
SHOX2 | Forward | ATCGCAAAGAGGATGCGAAAGGGA | 86 | 60 |
Reverse | TTCCAGGGTGAAATTGGTCCGACT | Â | Â | |
TBX3 | Forward | TTGAAGACCATGGAGCCCGAAGAA | 91 | 60 |
Reverse | CCCGCTTGTGAAACTGATCCCAAA | Â | Â | |
TBX18 | Forward | TTAACCTTGTCCGTCTGCCTGAGT | 147 | 60 |
Reverse | GTAATGGGCTTTGGCCTTTGCACT | Â | Â | |
TNNT2 | Forward | TTCACCAAAGATCTGCTCCTCGCT | 166 | 60 |
Reverse | TTATTACTGGTGTGGAGTGGGTGTGG | Â | Â |