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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Extracellular vesicles from hair follicle-derived mesenchymal stromal cells: isolation, characterization and therapeutic potential for chronic wound healing

Fig. 3

EVs batch-to batch analysis, western blotting and flow cytometry surface marker analysis. a Mean size of each EVs fraction for the different batches (n = 8). b Mode size of each EVs fraction for the different batches (n = 8). c Number of EVs compared to final cell count (n = 6). N.S. non-significant, *p < 0.05; **p < 0.005 between groups. d Western blotting analysis of EVs and non-EVs markers of three different batches for each cell type. e Multiplex bead-based flow cytometry assay for detection of EV surface markers (n = 3). f Comparison of EVs surface marker expression from (e): AT-P10K vs HF-P10K (I), AT-P100K vs HF-P100K (II), HF-P10K vs HF-P100K (III), and AT-P10K vs AT-P100K (IV). *p < 0.05; **p < 0.005; ***p < 0.001 between groups

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