Fig. 6
Post-transplantation analysis of the human markers in rd12 retina. A Expression of human pro- and anti-inflammatory genes, TNFα, CD3E, IL-2, IL-1β, IL-6 and CCL2, and IL-4 and IL-10, respectively, B neuroprotective genes, BDNF, GDNF, IGF, CNTF, EGF, FGF, and PDGF, C retinal genes, PMEL, CD24, LHX2, RPE65, SERPINF1, CRX, SIX3, OTX2, RCVRN, RHO, SIX6, GNL3, FABP7, NF200, ABCG2, PTK7, PNCA, β-CATENIN, NOTCH1, and RPS27A. D neurogenesis genes, PAX6, NESTIN, TUJ1, PROX1, CALRETININ, and CALBINDIN. All gene expression was normalized to GAPDH and β-ACTIN. Error bars represent the SEM of triplicate experiment (**p ≤ 0.01). E Immunohistochemical analysis of paraffin-embedded sections of the retina showing expression of human (RPE65, RHO, PKCα CALB1, and PAX6) proteins. The sections were countered stained with HNA (red). Blue and green colors represent the DAPI staining of the nuclei and human proteins, respectively. The right column shows enlarged merged images (over exposed) of only the area in the box to enhance the contrast. Scale bars represent 50 µm scale bars. (Magnification: ×40). F Measurement of fluorescent intensity of proteins as shown in E (**p ≤ 0.01). G Fluorescent intensity for the quantification of the number of HNA-positive cells in the RPE, ONL, INL and RGC layers of the retina (**p ≤ 0.01). H Percentage of dual-stained transplanted cells for HNA with RPE65, RHO, PKCα, CALB1, and PAX6 in each of the retina layers (**p ≤ 0.01)