Fig. 1
CHIR and IDE1 inefficiently induced DE differentiation from hPSCs. A Schematic of the two-step chemical strategy to differentiate hPSCs into DE cells. CHIR3: 3 µM CHIR99021, CHIR1: 1 µM CHIR99021. B Immunostaining analyzed SOX17, OCT4, and BRA expression in the differentiated cells. Scale bars 200 μm. C Overview of lineage relationships during DE differentiation from hPSCs. PS: primitive streak, PrE: primitive endoderm, MD: mesoderm, DE: definitive endoderm. D Quantitative RT-PCR analysis lineage relative genes expression of differentiated cells during DE induction. Data are presented as mean ± SEM, n = 3. E Immunostaining analyzed SOX17 and SOX7 expression in the differentiated cells. Scale bars 200 μm. F Quantitative RT-PCR analyzed signaling pathway-related genes expression patterns in DE induction with different protocols. Data are presented as mean ± SEM, n = 3