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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Efficiently generate functional hepatic cells from human pluripotent stem cells by complete small-molecule strategy

Fig. 2

Manipulating small-molecule cocktails to improve DE differentiation. A Schematic of the strategy to differentiate hPSCs into DE cells with small molecules. B Quantitative RT-PCR analysis lineage relative genes expression of differentiated cells during DE induction with different protocols. Data are presented as mean ± SEM, n = 3. C Immunostaining analyses of SOX17 and FOXA2 expression in DE cells derived from different protocols. Scale bars 200 μm. D The efficiency of SOX17 and FOXA2 expression after different cocktails treatment, determined by the FACS examination in differentiated cells

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