Fig. 7From: Efficiently generate functional hepatic cells from human pluripotent stem cells by complete small-molecule strategyRepopulation of mice injured liver by HBs transplantation. A Schematic diagram depicting HBs transplantation experimental schedule in immune-deficiency mice B Survival curve of mice. C Hematoxylin and eosin staining in mice liver. Scale bar 200 μM. D, E Engraftment of transplanted human HBs in mice liver after 1 week transplantation, as indicated by immunostaining of human ALB (green) and Di1 (red). Sham mice's liver as the negative control. Scale bar 100 μM. F Detection of AST and ALT levels in mice serum at one week after HBs transplantation. Data were analyzed by 2-tailed t tests. G After 4 weeks of cell transplantation, the reproduction of mice liver is indicated by immunostaining of human ALB (green) and Di1 (red). Scale bar 100 μM. H Flow cytometric analyses human ALB-positive hepatocytes in mice liver transplanted with human HBs. I Human ALB secretion in the mice serum. Data are presented as mean ± SEM, n = 4Back to article page