Fig. 1
From: Chemically defined and small molecules-based generation of sinoatrial node-like cells
Identification and characterization of SANLCs derived from hPSCs in a CDM3-based differentiation method. a Scheme of the CDM3-based differentiation protocol used for cardiomyocyte differentiation from hPSCs. b Relative expression of SHOX2, TBX18, HCN4, and TBX3 mRNA by using chemically defined cardiac differentiation protocol. Values represent expression levels relative to the housekeeping gene GAPDH (n = 4). c Representative flow cytometric analyses of the proportion of NKX2-5−cTNT+ and SHOX2+cTNT+ cells in CDM3-based differentiation method at day 24 (n = 3). d Representative action potential recordings using whole-cell patch of three cardiomyocyte subtypes produced from day 32 induced cells (total = 20 cells). e Histogram plot showing the distribution of the maximum upstroke velocities (dV/dtmax) recorded in 20 cardiomyocytes. SANLCs displayed slow maximum upstroke velocities (< 30 V/s), while WCMs displaying fast upstroke velocities (> 30 V/s). SANLCs, sinoatrial node-like cells; hPSCs, human pluripotent stem cells; WCMs, working cardiomyocytes; CHIR, CHIR99021, WNT signaling agonist; WNT-C59, WNT signaling inhibitor