Fig. 4
From: Chemically defined and small molecules-based generation of sinoatrial node-like cells
Enrichment of SANLCs with metabolic selection. a Scheme of SANLCs metabolic selection. b Heatmap shows expression changes of genes related to SAN, atrial cardiomyocyte, ventricular cardiomyocyte, atrioventricular node, pan-cardiomyocyte, and non-CMs cells at day 32 via qPCR. c Relative expression of SHOX2, HCN4, SCN3B and TNNT2 mRNA. Values represent expression relative to the housekeeping gene GAPDH. One-way ANOVA followed by Bonferroni’s post hoc test: *P < 0.05, **P < 0.01 versus untreated control or indicated sample (n = 4). d, e Representative flow cytometric analyses of PBC group following metabolic selection at day 32. *P < 0.05 by Student’s t test (n = 3). All error bars represent the SD of three or four independent experiments