Fig. 1From: MFN2 knockdown promotes osteogenic differentiation of iPSC-MSCs through aerobic glycolysis mediated by the Wnt/β-catenin signaling pathwayMFN2 knockdown promotes osteogenic differentiation of iPSC-MSCs. A The morphology of WT, MFN2-KD, and MFN2-OE iPSC-MSCs under optical and fluorescence microscope. B–C Western blot analysis of MFN2 in WT, MFN2-KD, and MFN2-OE iPSC-MSCs. D–E qRT-PCR analysis of MFN2 in WT, MFN2-KD, and MFN2-OE iPSC-MSCs. F–H qRT-PCR and Western blot analysis of osteogenic differentiation markers in WT, MFN2-KD, and MFN2-OE iPSC-MSCs after osteogenic differentiation. I Alizarin red staining of WT, MFN2-KD, and MFN2-OE iPSC-MSCs after osteogenic differentiation. RFP (red fluorescent protein) and GFP (green fluorescent protein) are the label proteins of lentivirus. NC: negative control. IM, osteogenic induction medium. ns, no significant. Data are expressed as mean ± standard deviation (SD), *P < 0.05, **P < 0.01, ***P < 0.001Back to article page