Fig. 4From: MFN2 knockdown promotes osteogenic differentiation of iPSC-MSCs through aerobic glycolysis mediated by the Wnt/β-catenin signaling pathwayWnt/β-catenin signaling pathway regulates glycolysis of iPSC-MSCs. A–B The relative basal glycolysis and glycolytic capacity of WT, MFN2-KD, MFN2-OE iPSC-MSCs, and cells treated with XAV-939 and SKL2001. C, F qRT-PCR analysis of glycolytic enzymes in WT, MFN2-KD, MFN2-OE iPSC-MSCs, and cells treated with XAV-939 and SKL2001. D, G Relative activity of glycolytic enzymes in WT, MFN2-KD, MFN2-OE iPSC-MSCs, and cells treated with XAV-939 and SKL2001. E, H Lactic acid levels of WT, MFN2-KD, MFN2-OE iPSC-MSCs, and cells treated with XAV-939 and SKL2001. I–J The relative basal and maximal respiration of WT, MFN2-KD, MFN2-OE iPSC-MSCs, and cells treated with XAV-939 and SKL2001. ns, no significant. Data are expressed as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001Back to article page