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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: MFN2 knockdown promotes osteogenic differentiation of iPSC-MSCs through aerobic glycolysis mediated by the Wnt/β-catenin signaling pathway

Fig. 5

MFN2 knockdown promotes aerobic glycolysis via the Wnt/β-catenin signaling pathway. A, B The Western blot analysis of β-catenin in WT, MFN2-KD, MFN2-OE iPSC-MSCs, and cells treated with XAV-939 and SKL2001 after 24 h of osteogenic induction. CD The relative basal glycolysis and glycolytic capacity of WT, MFN2-KD, MFN2-OE iPSC-MSCs, and cells treated with XAV-939 and SKL2001 after 14 days of osteogenic differentiation. E, G qRT-PCR analysis of glycolytic enzymes in WT, MFN2-KD, MFN2-OE iPSC-MSCs, and cells treated with XAV-939 and SKL2001 after 14 days of osteogenic differentiation. F, H Relative activity of glycolytic enzymes in WT, MFN2-KD, MFN2-OE iPSC-MSCs, and cells treated with XAV-939 and SKL2001 after 14 days of osteogenic differentiation. I The glucose consumption rate of WT, MFN2-KD, MFN2-OE iPSC-MSCs, and cells treated with XAV-939 and SKL2001 after 14 days of osteogenic differentiation. J Lactic acid levels of WT, MFN2-KD, MFN2-OE iPSC-MSCs, and cells treated with XAV-939 and SKL2001 after 14 days of osteogenic differentiation. K-L The relative basal and maximal respiration of WT, MFN2-KD, MFN2-OE iPSC-MSCs, and cells treated with XAV-939 and SKL2001 after 14 days of osteogenic differentiation. IM, osteogenic induction medium. ns, no significant. Data are expressed as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001

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