Fig. 6

MFN2 knockdown promotes osteogenic differentiation through aerobic glycolysis via the Wnt/β-catenin signaling pathway. A–B qRT-PCR analysis of osteogenic differentiation markers in WT, MFN2-KD, MFN2-OE iPSC-MSCs, and cells treated with XAV-939 and SKL2001 after 14 days of osteogenic differentiation. C–D Western blot analysis of osteogenic differentiation markers in WT, MFN2-KD, MFN2-OE iPSC-MSCs, and cells treated with XAV-939 and SKL2001 after 14 days of osteogenic differentiation. E Alizarin red staining of WT, MFN2-KD, MFN2-OE iPSC-MSCs, and cells treated with XAV-939 and SKL2001 after 14 days of osteogenic differentiation. F The interaction between MFN2, β-catenin, aerobic glycolysis, and osteogenic differentiation. IM, osteogenic induction medium. ns, no significant. Data are expressed as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001