Fig. 8

MFN2 knockdown promotes aerobic glycolysis via the Wnt/β-catenin signaling pathway in PDLSCs. A–B The Western blot analysis of β-catenin in WT, MFN2-KD, MFN2-OE PDLSCs, and cells treated with XAV-939 and SKL2001 after 24 h of osteogenic induction. C–D The relative basal glycolysis and glycolytic capacity of WT, MFN2-KD, MFN2-OE PDLSCs, and cells treated with XAV-939 and SKL2001 after osteogenic differentiation. E, G qRT-PCR analysis of glycolytic enzymes in WT, MFN2-KD, MFN2-OE PDLSCs, and cells treated with XAV-939 and SKL2001 after osteogenic differentiation. F, H Relative activity of glycolytic enzymes in WT, MFN2-KD, MFN2-OE PDLSCs, and cells treated with XAV-939 and SKL2001 after osteogenic differentiation. I The glucose consumption rate of WT, MFN2-KD, MFN2-OE PDLSCs, and cells treated with XAV-939 and SKL2001 after osteogenic differentiation. J Lactic acid levels of WT, MFN2-KD, MFN2-OE PDLSCs, and cells treated with XAV-939 and SKL2001 after osteogenic differentiation. K–L The relative basal and maximal respiration of WT, MFN2-KD, MFN2-OE PDLSCs, and cells treated with XAV-939 and SKL2001 after osteogenic differentiation. IM, osteogenic induction medium. ns, no significant. Data are expressed as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001