Fig. 2

BCAS2 cKO mice show reduced number and proliferation of Sox2⁺ NSCs. A The scheme for Sox2⁺NSC proliferation analysis. BrdU administration on day 11 for 3 days in mice at age 6 weeks. B Representative images of Sox2⁺BrdU⁺Cre⁺ cells in SGZ by confocal microscopy. Scale bar 100 μm. Quantification of Ba Sox2⁺BrdU⁺ cells, Bb proportion of Sox2⁺BrdU⁺/Sox2⁺ cells, Bc Cre⁺Sox2⁺BrdU⁺ cells, and Bd proportion of Cre⁺Sox2⁺BrdU⁺/Cre⁺ cells in SGZ. Arrows: Cre⁺Sox2⁺BrdU⁺ Arrowheads: Sox2⁺. C Representative images of Ki67⁺ expression in dentate gyrus (left). Scale bar: 100 μm. Arrows: Ki67⁺. Right: quantification of Ki67⁺ cells in SGZ. Data are mean ± SEM by Student’s t test. *P < 0.05; **P < 0.01