Fig. 5

Reduced β-catenin expression in hippocampus of BCAS2-knockdown mice. A Representative images of GFP expression in cortex and hippocampus at 2 weeks after lentivirus injection in hippocampal dentate gyrus (shown in Fig. 4A). Scale bar: 50 μm. B Western blot analysis of BCAS2 and β-catenin protein levels with lysates from cortex and hippocampus under mock and shBCAS2 treatment. Quantification of relative β-catenin protein level in cortex (Ba) and hippocampus (Bb). N = 6 for each group. C Representative staining of GFP⁺ and β-catenin. Arrows: GFP⁺ cells (BCAS2-knockdown cells) showing low expression of β-catenin. Arrowheads: GFP⁻ cells (non-infected cells) showing high expression of β-catenin. Scale bar: 50 μm. Right: quantification of β-catenin expression intensity in GFP⁺ and GFP⁻ cells in shBCAS2 mice. Ten single cells for each mouse were counted from three separate experiments. Data are mean ± SEM by Student’s t test. **P < 0.01, ns not significant