Fig. 9

Intracranial hippocampal injection of AAV-β-catenin in BCAS2 cKO mice could rescue adult neurogenesis. A Western blot analysis of hippocampus in AAV-β-catenin at 2 weeks after intracranial injection. B Representative images of Flag (β-catenin) and Sox2 in hippocampal dentate gyrus (DG) of cKO-GFP and cKO-β-catenin mice. Scale bar: 100 μm. Quantification of Ca Sox2⁺, Cb GFP⁺ Sox2⁺/GFP⁺ and Cc BrdU⁺ cells in SGZ. D Quantification of Ki67⁺ cells in SGZ. E Representative images of DCX, GFP, and Flag expression. Scale bar: 100 μm. Quantitation of Ea DCX⁺/DG and Eb GFP⁺DCX⁺ (for cKO-GFP) or Flag⁺ DCX⁺ cells (for AAV-β-catenin) in DG. Data are mean ± SEM by Student’s t test, *P < 0.05; **P < 0.01; ***P < 0.005