Fig. 6
MB-MSCs transplanted i.v. or i.p. suppress Th1 and Th17 cell responses. EAE was induced in mice and 6 days later, P4 MB-MSCs were transplanted via i.v. (i.v.) or i.p. (i.p.) route. Mice that received only PBS served as untreated controls. On day 7, CD4 T cell responses were analyzed in spleen. a The transcription factors T-bet and RORγt were stained and percentages of T-bet and RORγt-expressing CD4+ cells were plotted and compared. Pool of four experiments with two mice per group per experiment (n = 8). b Intracellular cytokine staining was performed after MOG35-55 peptide restimulation, and percentages of IFNγ, IL-17, GM-CSF, and CD40L-expressing cells were plotted and compared. Pool of three experiments with two mice per group per experiment (n = 6). c Mice were transplanted i.v. with P4 MB-MSC or PBS. One day later, EAE was induced, and 5 weeks later, CD4+ T cell responses were analyzed in spleen after MOG35-55 peptide restimulation. Percentages of TNFα, IFNγ, IL-17, and GM-CSF-expressing cells were plotted and compared. Pool of three experiments with two mice per group per experiment (n = 6)